【摘要】 采用植物组织培养手段建立了稳定的黄芩细胞悬浮系,通过正交试验筛选出最佳的MS液体培养基为:NH4+/NO3-30/40 mmol/L,Ca2+3 mmol/L,pH值5.4,蔗糖3%。研究了碳源、肌醇、激素、水解酪蛋白(CH)和水解乳蛋白(LH)对悬浮系中黄芩甙含量的影响。结果表明:复合碳源、较低浓度肌醇对悬浮细胞系中黄芩甙的积累和胞外释放具有促进作用,2.0 mg/L NAA+0.5 mg/L Kt最有利于悬浮系中黄芩甙的积累;水解酪蛋白和水解乳蛋白对黄芩甙的生成没有促进作用,反而起到了一定的抑制效应。更多还原

【Abstract】 The stable cell suspension cultures of Scutellaria baicalensis Georgi was obtained by plant tissue culture.The optimum liquid MS medium was NH4+/NO3-30/40 mmol/L,Ca2+ 3 mmol/L,pH 5.4,sucrose 3%.The effects of carbon source,inositol,plant hormone,casein hydrolysate(CH)and lactalbumin hydrolysate(LH)on baicalin content in cell suspension cultures were studied.Results showed that carbon source and low concentration inositol had positive effect on the accumulation as well as the outside release of baicalin in cell suspension cultures.2.0 mg/L NAA and 0.5 mg/L Kt was fit for baicalin accumulation in cell suspension cultures.CH and LH had no facilitation but some depressing effects on the formation of baicalin.更多还原