【摘要】 以驱蚊香草的幼嫩叶片为外植体,应用正交设计法对愈伤组织诱导条件进行研究。结果表明:诱导愈伤组织的最佳培养基配方为:MS+2,4-D 2mg/L+6-BA 0.5mg/L+IBA 0.2mg/L;外植体用10%的84消毒液消毒10min效果最好;最适培养温度为20℃,最佳光照时间为10h/d。更多还原

【Abstract】 With the orthogonalty design,the young leaf of citrosa was used for tissue culture and the induction condition of callus was studied.The result showed that the optimum medium for callus was MS + 2,4-D 2 mg/l + 6-BA 0.5 mg/l + IBA 0.2 mg/l and when the explant was disinfected with 10% of 84 Disinfectant Liquid,it had a better result.The optimum temperature was 20 ℃ and the best light hour was 10 h/d.更多还原