Factors in rhizome differentiation of Cymbidum niveo-maginatum were studied in this paper.The results showed that shoot formation was favorable in Hyponex-2(N∶P∶K=20∶20∶20)medium and mass shoots were found in this medium.Moreover,BA 4 mg/L in Hyponex-2 medium and 400 lx of light intensity promoted shoot formation of Cymbidum niveo-maginatum.During rooting stage,NAA 1.0 mg/L was suitable for rooting and shoot growth.