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小鼠生物量定性检测法检测细菌发酵产生的河豚毒素
Application of Mouse-biodetection Method in Detecting Tetrodotoxin Fermented by Bacteria
【摘要】 建立了ICR品系小鼠生物量定性检测河豚毒素的标准曲线,并将其应用于细菌发酵液中河豚毒素的检测中,取得较理想的结果,冷冻干燥后得到海藻希瓦氏菌,其菌体部分的产毒力为2.06μg/ml,菌液部分为1.53μg/ml。
【Abstract】 The standard curve of mouse-biodetection method was founded based on ICR mouse,and was used to detect TTX at fermentation solution of Shewanella alga.And an ideal result had been got.After being lyophilized,2.06 μg/ml and 1.53 μg/ml virulence were detected from S.alga cells and fermentation solution.
【基金】 山东省教育厅资助项目(001164504)
- 【文献出处】 安徽农业科学 ,Journal of Anhui Agricultural Sciences , 编辑部邮箱 ,2007年02期
- 【分类号】Q936
- 【被引频次】7
- 【下载频次】219