【摘要】 采用非手性的C18色谱柱,通过在流动相中加入手性选择剂β-环糊精和在样品中加入β-环糊精的方法实现对盐酸西布曲明对映体的拆分。流动相组成为含β-环糊精的甲醇-水(50∶50,V/V,pH=6)。当手性选择剂β-环糊精的浓度为0.8mmol/L时,盐酸西布曲明对映体在进样后的5.7min得到了基线分离。更多还原

【Abstract】 By adding β-cyclodextrin as chiral selector into mobile phase and in Sibutramine hydrochloride ,sibutramine hydrochloride enantiomers were separated using non-chiral C18 column in high performance liquid chromatography(HPLC).In the mobile phase composed of methano-H2O(50∶50,V/V ) containing 0.8 mmol/L β-cyclodextrin at pH=6,the two enantiomers were well separated in 5.7 minutes.The mechanism of chiral separation through adding chiral selector into mobile phase was also discussed.更多还原