【摘要】 背景与目的:将瑞-姬(Wright-Giemsa)氏混染法用于Cr(Ⅵ)诱导的HepG2细胞微核试验,探讨瑞-姬氏混染配比浓度和不同染色时间对染色效果的影响。材料与方法:以瑞-姬氏混染对HepG2细胞进行染色,采用不同的染液配比和染色时间,观察其对染色效果的影响。结果:3∶1瑞-姬氏混染3~5min可以获得较瑞氏或姬氏单一染色更好的染色效果。结论:瑞-姬氏混合染色法可应用于微核试验中的细胞染色,并可获得良好的染色效果。更多还原

【Abstract】 BACKGROUND & AIM: To explore the influence of different concentrations and different proportions of Wright-Giemsa dye on its staining effect, through application to micronucleus test of HepG2 cells induced by hexavalent chromium[Cr(Ⅵ )]. MATERIALS AND METHODS: HepG2 cells were stained with different proportions of Wright-Giemsa dye and for different stain time, and their influence on staining effects were assessed. RESULTS: Better results could be obtained when cells were stained with a concentration of Wright-Giemsa at 3∶ 1 for 3-5 minutes. CONCLUSION: Wright-Giemsa stain could be applied to micronucleus test with favorable effects.更多还原