【摘要】 背景与目的:研究两种人类来源的肝细胞在评价饮水氯化消毒副产物3-氯-4-二氯甲基-5-羟基-2(5氯)-呋喃酮(MX)DNA损伤作用的敏感性。材料与方法:应用单细胞凝胶电泳试验(彗星试验),研究人肝癌细胞株HepG2和人胚肝细胞L-02在评价饮水氯化消毒副产物MXDNA损伤作用的敏感性。结果:随着MX剂量增加,可引起HepG2(>30μmol/L)和L-02细胞(>100μmol/L)DNA迁移度显著增加;中度以上损伤所占百分比在同剂量组中HepG2细胞大于L-02细胞,经比较分析,差异有统计学意义(χ2=20.985;8.0;11.97,P<0.05)。结论:应用单细胞凝胶电泳试验评价MX的DNA损伤作用,人肝癌细胞株HepG2比人胚肝细胞L-02更为敏感。更多还原

【Abstract】 BACKGROUND &AIM: To study on the susceptibility of two human-derived hepatic cell lines in evaluating DNA damage induced by 3-chloro-4-dichloromethyl-5-hydroxy-2(5H)-furanone(MX). MATERIALS AND METHODS: Using single cell gel electrophoresis assay, to evaluate the DNA damage induced by MX in human hepatocellular carcinoma cell lines(HepG2) and human fetal hepatocytes (L-02). RESULTS: MX could induce HepG2 and L-02 DNA single strand breaks at the concentrations of 30 and 100 μmol/L, respectively. The length of DNA migration induced by MX increased significantly in comparison to solvent control (DMSO) in a dose-response manner(P<0.05). Moderate or greater DNA damages induced by MX in HepG2 were more serious than that of L-02(χ2=20.985;8.0;11.97,P<0.05). CONCLUSION: HepG2 was more susceptible than L-02 cells to DNA damage induced by MX evaluated by using single cell gel electrophoresis assay.更多还原