【摘要】 目的检测IL-12和孕酮在多发性硬化(MS)患者中的水平,探讨男、女性多发性硬化患者存在的发病机制,比较男、女性MS患者在临床上发病的差异。方法采用酶联免疫(ELISA)法检测60例MS患者的脑脊液和40例患者血液中IL-12的水平,应用放射免疫法测定40例MS患者血液中孕酮的含量,并与40例正常对照组比较(以上标本男女各半)。结果男、女性MS患者血液中和脑脊液IL-12的含量明显高于正常对照组(P<0.01);女性患者血液中孕酮的含量明显低于正常对照组(P<0.01),男性患者血液中孕酮的含量与正常对照组差别不明显(P>0.05);女性患者血液中孕酮对IL-12的含量存在负相相关作用(r=-0.80,P<0.01),男性相关作用不明显(r=-0.38,P>0.05)。结论IL-12参与多发性硬化的发病过程;孕酮可能是男、女性患者发病差异性的原因之一。更多还原

【Abstract】 Objective To detect the contents of IL-12 and progesterone and probe into the pathogenesis in the patients and compare the difference between male and female patients with multiple sclerosis.Methods IL-12 in the CSF with 60 patients and blood serum with 40 patients was assessed by enzyme-linked immunosorbent assay (ELISA) and progesterone in the blood serum with 40 patients by radio-immunity(RI).We compared them with the 40 control subjects and half of them were male patients.Results The levels of IL-12 in CSF and blood serum were significantly higher in the patients than those in the controls(P< 0.01); The progesterone of blood serum in the female patients was significantly lower than that in the control(P< 0.01). There was no significant difference in the progesterone level between the male patients and the controls(P> 0.05). There was negative correlation between progesterone and IL-12 in the blood serum of female patients (r= -0.80,P< 0.01).The correlation about male patients is not obvious(r= -0.38,P> 0.05)。Conclusions IL-12 may have an important rate in the pathogenesis of MS and progesterone may be one of the causes of difference between males and female patients.更多还原