【摘要】 目的探讨小柴胡汤对子宫内膜异位症（内异症）大鼠异位内膜的影响及其作用机制。方法将内异症模型大鼠,分为5组,小柴胡汤低剂量组(5g·kg^-1·d^-1,n=10)、小柴胡汤中剂量组(10g·kg^-1·d^-1,n=10)、小柴胡汤高剂量组(15g·kg^-1·d^-1,n=10)、丹那唑组(0．1g·kg^-1·d^-1,n=9)及模型对照组(20ml·kg^-1·d^-1蒸馏水,n=10),连续给药4周后,利用透射电镜技术及免疫组化的方法,观察小柴胡汤对内异症大鼠异位内膜的影响；分析异位内膜Fas及Caspase-3蛋白的表达。结果小柴胡汤各组异位内膜生长明显受抑制（P＜0．05,P＜0．01）；异位内膜Fas蛋白、Caspase-3蛋白的表达水平明显高于其在位内膜。结论小柴胡汤能抑制内异症大鼠异位内膜的生长,其作用机制可能是通过上调Fas蛋白的表达,促进异位内膜细胞的凋亡而实现的。更多还原

【Abstract】 Objective To explore the effect of Xiaochaihu Decoction（XCHD）on ectopic endometrium in rats with endometriosis and its mechanism.Methods The rat model of endometriosis was established and rats were divided into 5 groups,the low dosage of XCHD group(5g·kg^-1·d^-1,n=10),the medium dosage of XCHD group(10g·kg^-1·d^-1,n=10),the high dosage of XCHD group(15g·kg^-1·d^-1,n=10),the danazol group(0.1g·kg^-1·d^-1,n=9)and the control group(20 ml·kg^-1·d-^-1 distilled water,n=10).Af- ter treated for four successive weeks,the effect of XCHD on eetopic endometrium was evaluated by observing the changes in transmission electron microscopy.Fas and Caspase-3 protein expression in endometrium and endometri- otic tissue were observed by immunohistoehemistry method.Results Ectopic endometrial growth was markedly in- hibited（P＜0.05 or P＜0.01）in all the XCHD groups;Fas and Caspase-3 protein expressions of ectopic endom- etrial tissue were higher than those in the endometrium.Conclusion XCHD could inhibit the growth of the eetop- ic endometrium in rats with experimentally induced endometriosis.The therapeutic mechanism may be related to promoting the apoptosis in ectopic endometrial tissue by increasing Fas protein expression.更多还原