【摘要】 目的观察银杏叶提取物（金钠多）预处理对豚鼠—大鼠非协调性异种心脏移植物的影响并探讨其作用机理。方法采用颈部异位心脏移植模型,分4组：1组（眼镜蛇毒因子预处理,n=10）；2组（眼镜蛇毒因子+金钠多预处理,n=5）；3组（眼镜蛇毒因子+环孢素A预处理,n=8）；4组（眼镜蛇毒因子+环孢素A+金钠多预处理,n=8）。结果各组移植物病理表现均为急性血管性排斥反应（AVR）改变。移植物平均存活时间分别为1组41 h、2组68 h、3组55 h、4组74 h。金钠多预处理的移植物细胞间黏附分子-1 （ICAM-1）表达减少（P＜0．05）,金钠多联合环孢素A预处理的移植物CD₆₈和CD₅₇表达下调,血红素氧合酶-1 （HO-1）表达上调,凋亡指数降低。结论银杏叶提取物预处理可延长非协调性异种移植物存活时间,与环孢素A伍用可明显减轻异种AVR损害。更多还原

【Abstract】 Objective To investigate the effect of ginkgo biloba extract（ginaton）preconditioning on discord- ant cardiac xenografts from guinea pig to rat,and explore its mechanism.Methods Cervical cardiac transplanta- tion model was established in the rats,which were divided into 4 groups:Group 1[cobra venom factor（CVF）pre- treatment,n=10];Group 2（CVF＋ginaton,n=5）;Group3[cyclosporine（CsA）;Group 4(CVF＋CsA＋gi- naton,n=8].The survival time and histopathology after xenograft were observed and expressions of intercellular ad- hesion molecule-1（ICAM-1）,heme oxygenase-1（HO-1）,CD₆₈and CD₅₇were detected.Results Pathologic man- ifestion of grafts showed changes of acute vascular rejection（AVR）in all groups.The mean survival time after car- diac xenograft was 41 hrs in Group 1,68 hrs in Group 2,55 hrs in Group 3 and 74 hrs in Group 4.Expression of intercellular adhesion molecule-1（ICAM-1）decreased after ginaton preconditioning（P＜0.05）.CD₆₈and CD₅₇ex- pressions were down-regulated,HO-1 expression was up-regulated,as well as the apoptotic index（AI）reduced sig- nificantly after ginaton with cyclosporine A preconditioning.Conclusion Ginaton preconditioning can prolong the survival time after discordant xenograft,and significantly alleviate pathological lesion from acute xenograft vascular rejection combined with eyclosporine A.更多还原