【摘要】 目的观察中药胃康宁含药血清对胃癌细胞生长和VEGF及其受体表达的作用。方法分别以高、中、低等不同剂量的胃康宁制剂对Wistar雄性大鼠进行灌胃,制备含药血清,然后分别用不同剂量组合药血清培养胃癌细胞,以倒置显微镜及流式细胞仪观测各组胃癌细胞的生长状况,免疫组织化学SABC法检测各组胃癌细胞中血管内皮生长因子（vascular endothelial growth factor,VEGF）及其受体Flt-1的表达,RT- PCR法检测各组胃癌细胞中VEGF及其受体KDR,Flt-1 mRNA的表达。结果与对照组比较,用药组胃癌细胞的生长及细胞周期明显改变（P＜0．01）,用药组G₀-G₁期细胞增多,S期细胞减少,凋亡率增加,并呈一定的量效关系。VEGF的灰度值由对照组的147．82±0．15升高到低剂量组的174．80±0．81,中剂量组的178．65±0．56,高剂量组的182．44±0．54（P＜0．01）。Flt-1的灰度值由对照组144．31±0．71升高到低剂量组148．20±0．69,中剂量组162．01±0．52,高剂量组168．51±0．81（P＜0．01）。胃康宁含药血清培养的胃癌细胞中VEGF及其受体KDR、Flt-1 mRNA的表达明显减弱（P＜0．01）。结论中药胃康宁对胃癌细胞的生长,VEGF及其受体的表达有抑制作用。更多还原

【Abstract】 Objective o observe the effect owf drug-containing serum of Chinese traditional herbal decoction Weikangning（WKN）on growth of gastric cancer cell,expression of vascular endothelial growth factor（VEGF） and its receptors including KDR and Fit-1.Methods A total of 120 male Wistar rats were given high,medium and low-dose WKN.After the drug-containing serum was prepared,the gastric cancer cells MGC-803 of different dose groups were cultured with the drug-containing serum,respectively.The gastric cell growth was observed by using light microscope and flow cytometer,the expression of VEGF and its receptor Flt-1 was detected with SABC immunohistochemistry method and the mRNA expression levels of VEGF and its receptors including KDR and Fh- 1 of different groups were detected with reverse transcription-polymerase chain reaction（RT-PCR）,respectively. Results The gastric cancer cell growth and cell cycle of the three medicated groups were significantly improved as compared with those of the control group（P＜0.01）,the proportion of cells in G₀-G₁ phase was increased,while the cells in S phase were decreased.It was shown that the apoptotic rates were increased in the medicated groups in a dose-dependent manner.The gray scales（μm²）of VEGF and Flt-1 in high,medium and low dose groups was 182.44±0.54,178.65±0.56,174.80±0.81 and 168.51±0.81,162.01±0.52,148.20±0.69,respectively vs 147.82±0.15（P＜0.01）and 144.31±0.71（P＜0.01）in the control group.The mRNA expression of VEGF and its receptors significantly decreased in gastric cancer cells after cultured with WKN contained serum （P＜0.01）.Conclusion WKN has inhibitory effects on gastric cancer cell growth and mRNA expression of VEGF as well as its receptors KDR and Fit-1.更多还原