节点文献

黑根霉对甾体的C₁₁α羟基化反应

STUDIES ON THE REACTION OF STEROID C₁₁α-HYDROXYLATION BY RHIZOPUS NIGRICAN

推荐 CAJ下载
PDF下载
不支持迅雷等下载工具，请取消加速工具后下载。

【Author】 DU Da-qing~1,LIU Ling-ling~1,ZHANG Xing-yuan~1,FENG Wen-zhong~2(1.The Key Laboratory of Industrial Biotechnology,Ministry of Education,Southern Yangtze University,Wuxi 214036,China;2.Henan Lihua Pharmaceutical Co,Ltd,Anyang 455000,China)

【机构】江南大学生物工程学院工业生物技术教育部重点实验室；河南利华制药有限公司江苏无锡214036；江苏无锡214036；河南安阳455000；

【摘要】从黑根霉(Rhizopus nigricans)对16α,17α环氧黄体酮的C11α羟基化反应发酵过程曲线开始,考察了碳源、pH对黑根霉的甾体羟基化转化过程的影响.100 L发酵罐实验结果表明,采用复合碳源C(葡萄糖∶糊精为1∶1),转化率比对照提高2%.100 L发酵罐实验结果表明,氧化期(从投料开始计)调pH至4.0~5.0,转化率提高3%.更多还原

【Abstract】 The effects of carbon source and pH on C₁₁αhydroxylation of 16α,17αepoxy4pregnene3,20-dione were studied basing on the figure of steroid C-（11）αhydroxylation by Rhizopus nigricans.The 100 L bioreactor conversion rate was increased by 2% when using complex carbon source C（glucose:dextrin=1:1）.By adjusting pH to（4.0～5.0） in oxidation phase the 100 L bioreactor conversion rate was increased by 3%.更多还原