【摘要】 目的：探讨三氧化二砷（As₂O₃）对胃癌细胞SGC7901/ADR阿霉素（ADM）耐药性的逆转作用及其逆转机制。方法：采用MTT法检测As₂O₃的非细胞毒性浓度和SGC7901/ADR细胞对ADM的敏感性,用流武细胞仪检测细胞内药物浓度和P-gp功能,免疫组织化学法检测细胞GST-π和TPPOⅡ的表达。结果：0．4～0．8μmol/L As₂O₃对耐药细胞SGC7901/ ADR无明显毒性,0．8μmol/L的As₂O₃可抑制P-gp功能,下调GST-π表达,显著提高SGC7901/ADR细胞内ADM浓度,增加SGC7901/ADR细胞对ADM的敏感性。结论：As₂O₃可部分逆转SGC7901/ADR细胞对ADM耐药性,其机制可能与抑制P-gp功能及下调GST-π表达有关。更多还原

【Abstract】 Objective:To explore the reversal effect of arsenic trioxide （As₂O₃） on the multidrug resistance of human gastric carcinoma SGC7901/ADR cell line to adriamycin（ADM）and its reversal mechanisms.Methods:The non-cytotoxic concentrations of As₂O₃ and the sensitivity to ADM in SGC7901/ADR cell line were detected by MTT assay.The drug concentration and P-gp function in SGC7901/ADR cell line were measured with flow cytometry（FCM）,and the impact of As₂O₃ on the expressions of GST-πand TOPOⅡin SGC7901/ADR cells was assessed by immunohistochemicai methods.Results:As₂O₃ at 0.4μmol/L to 0.8μmol/L concentrations was not significantly cytotoxic to SGC7901/ADR cell line.As₂O₃ could improve the sensitivity of SGC7901/ADR cell line to ADM via inhihiting P-gp function,down-regulating GST-πexpression and increasing the intracellular accumulation of ADM in SGC7901/ADR cell line at 0.8μmol/L concentration.Conclusions:As₂O₃ is able to partly reverse the re- sistance of SGC7901/ADR cell line to ADM,which may be related to inhibiting the P-gp function and down-regulating of GST-πexpression.更多还原