【摘要】 目的：研究c-kit受体(c-kit receptor,c-kit R)在急性白血病(acute leukemia,AL)的表达,探讨c-kit R的功能是否异常。方法：运用流式细胞术(flow cytometry,FCM)分别检测92倒急性淋巴细胞白血病(acute lymphoblastic leukemia,ALL)和81例急性非淋巴细胞白血病(acute nonlymphoblastic leukemia,ANLL)患者骨髓单核细胞(bone marrow mononuclear cell,BMMNC)跨膜c-kit R表达的阳性率和阳性水平；采用干细胞因子(stem cell factor,SCF)、重组人白介素3(recombinant hu- man interIeukin-3,rhIL-3)和重组人红细胞生成素(recombinant human erythropoietin,rhEPO)三种造血生长因子(hematopoi- etic growth factor,HGF)共培养4例正常人和8例ANLL组患者BMMNC,二组分别设HGFs培养前的标本为对照组,运用FCM分别检测正常人和ANLL二组的对照组以及HGFs培养后第4、8和12小时3个时间点的c-kit R阳性率和阳性水平。结果：①ANLL组患者c-kit R表达的阳性率及阳性水平均值分别为71.6%和(45.3±16.50)%,均明显高于正常人和ALL组患者(P值分别＜0.001)；②4例正常人对照组(HGFs培养前)的c-kit R阳性水平均值为(3.90±0.99)%；HGFs培养后第4、8和第12小时的c-kit R阳性水平分别为(5.8±4.05)%,(7.3±6.10)%和(8.3±5.20)%,与对照组之间无统计学意义的差异(P＞0.05),8例ANLL对照组(HGFs培养前)的c-kit R阳性水平的均值为(45.6±15.08)%,HGFs培养后三个时间点的c-kit R阳性水平的均值分别为(6.1±2.78)%、(5.8±2.58)%和(5.5±2.48)%,都低于对照组(P分别＜0.001),c-kit表达呈总体下调趋势。结论：c-kit R可作为AL的髓系免疫学标志物；HGFs对ANLL患者c-kit R的调控异常,提示在ANLL高表达的c-kit R的功能异常。更多还原

【Abstract】 Objective:To explore the expression of c-kit receptor in the patients with acute nonlymphoblastic leukemia(AN- LL)and further determine whether it has normal function.Methods:Flow cytometry analysis(FCM)was used to detect the pos- itive rate and positive level of transmembrane c-kit receptor expression in bone marrow mononuclear cells from 92 patients with a- cute lymphoblastic leukemia(ALL)and 81 patients with ANLL.Bone marrow mononuclear cells from 4 healthy controls and 8 ANLL patients were co-cultured with three hematopoietic growth factors(HGF).They are stem cell factor(SCF),recombinant human interleukin-3(rhIL-3),and recombinant human erythropoietin(rhEPO).The positive level of c-kit receptor before and at 4,8,and 12 h after co-culture with HGF were detected by FCM.Results:①The positive rate and average positive level of c-kit receptor expression in 81 patients with ANLL was 71.6% and(45.3±16.5)%,respectively,higher than those in healthy con- trois and patients with ALL(all P＜0.001).②The average positive level of c-kit receptor expression in 4 healthy controls be- fore HGFs co-culture was (3.90±0.99)% which had no significant difference with those measured at 4,8,and 12 h after HG- Fs co-culture(P=0.185).The average positive level of c-kit receptor expression in 8 ANLL patients before HGFs co-cuhure was (45.6±15.08)%.The expressions of c-kit receptor were at 4,8,and 12 h after HGFs co-culture were(6.10±2.78)%,(5.80±2.58)%,and(5.50±2.48 )%,respectively,which were all significancy lower than control group(all P＜0.001). It was down regulated gradually.Conclusion:The c-kit receptor has the potential to be the myeloid immunological marker for acute leu- kemia.The abnormal regulation of c-kit receptor by HC-Fs suggests that c-kit receptor highly expresses in ANLL patients that has abnormal function.更多还原