【摘要】 目的研究活血药丹参素和川芎嗪对血管紧张素Ⅱ(AngⅡ)诱导的心肌细胞凋亡的影响,探讨其抑制心肌肥大的作用机制。方法收集各组心肌培养细胞,PI染液,于流式细胞仪上进行检测。结果AngⅡ能诱导心肌细胞凋亡,AngⅡ组与空白对照组相比,心肌细胞凋亡率显著增加(P<0.01),并随着时间的延长其凋亡率不断增高,于第7天达到高峰;洛沙坦明显抑制AngⅡ所诱导各个时间段的心肌细胞凋亡率(P<0.01),而活血药丹参素和川芎嗪也明显抑制AngⅡ所诱导各个时间段的心肌细胞凋亡率(P<0.05或P<0.01)。结论丹参素和川芎嗪能通过抑制AngⅡ诱导的心肌细胞凋亡而产生心肌保护作用,具有防止心肌细胞肥大作用,从而防治心室肥厚。更多还原

【Abstract】 Objective To study the biological mechanisms of Danshensu and Ligustrazine for preventing apoptosis of cardiac hypertrophy induced by angiotensinⅡ (AngⅡ) .Methods Wistar rats were divided into control group,AngⅡgroup,AngⅡ+Losartan group,AngⅡ+ Danshensu group; AngⅡ+ Ligustrazine group. The cardiac cell of rats in each group were cultured in PI,and then was detected by colter EPICS XL.Results AngⅡinduced apoptosis.Losartan,Danshensu and Ligustrazine inhibited increase of the apoptosis rate of myocardial cells induced by AngⅡsignificantly (P<0.01 or P<0.05).Conclusion The results showed that Danshensu and Ligustrazine could inhibit apoptosis of myocardiac cells induced by AngⅡ,inhibit hypertrophy of myocardiac myocytes and prevent LVH.更多还原