【摘要】 目的建立运用荧光原位杂交技术(fluorescence in situ hybridization,FISH)检测未培养羊水细胞染色体异常的方法。方法对24例未培养羊水细胞进行FISH检测,其中用21号染色体荧光探针检测19例,X/Y染色体探针检测3例,13、18号染色体探针各检测1例。结果24例产前诊断者未培养羊水细胞检测出一例21三体。X/Y染色体探针检测时1例为2个绿色的X信号,2例均为1个红色Y信号和1个绿色X信号。检测结果与培养后羊水细胞检测结果一致,并与外周血染色体核型检测结果相符。结论本研究方法快速、简便、准确可靠,适于临床运用推广。更多还原

【Abstract】 Objectve: To study the method of the fluorescent in situ hybridization on detection of chromosomes abnormal in uncultured amniotic fluid. Methods:24 cases of uncultured amniotic fluid were studied,19 cases were detected by 21 probe chromosome,3 cases were detected by X/Y probe chromosomes,2 cases were detecded by 13、18 probe chromosome respectively. Results:One 21 trisom was observed in 24 cases.1 case was detected two green X signal and 2 cases were detected one green X signal and one red Y signal with X/Y probes.The results were confirmed by that of cultured amniotic fluid and chromosome analysis of cultured peripheral blood. Conclusion:The method is rapid and accurate, suitable for clinic.更多还原