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内皮型一氧化氮合酶运输介导物在子痫前期患者脐血管中的表达

The study of endothelial nitric oxide synthase traffic inducer in the umbilical vessels of The Patients with Pre-eclampsia

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【作者】 相文佩陈汉平沈红玲郭玉珍

【Author】 XIANG Wen-pei,CHEN Han-ping,SHENG Hong-ling,GUO Yu-zhen.(Department of Obstetrics and Gynecology,Tongji Hospital,Tongji Medical college,Huazhong Science and Technology University,Wuhan 430030,China)

【机构】 华中科技大学同济医学院计划生育研究所华中科技大学同济医学院附属同济医院妇产科华中科技大学同济医学院附属同济医院妇产科 430030430030

【摘要】 目的研究内皮型一氧化氮合酶运输介导物(endothelial n itric oxide synthase traffic inducer,NOSTR IN)在子痫前期(pre-ec lampsia,PE)患者脐血管中的表达,探讨其在子痫前期发病中的作用。方法免疫组织化学法检测脐血管中NOSTR IN的表达,并通过高清晰度彩色病理图文分析系统对其定量分析;RT-PCR检测子痫前期患者脐血管组织中NOS-TR IN mRNA的表达。结果免疫组织化学显示正常妊娠和子痫前期患者脐血管中都有NOSTR IN的表达,但子痫前期患者脐血管内皮细胞胞浆染色为深棕色,而正常妊娠者脐血管内皮细胞胞浆染色为浅棕色;RT-PCR显示子痫前期患者脐血管组织中NOSTR IN mRNA的表达水平显著高于正常妊娠(P<0.01)。结论子痫前期患者脐血管组织中NOSTR IN表达增加,这可能是子痫前期发病机制的重要因素之一。

【Abstract】 Objective:To investigate the expression of endothelial nitric oxide synthase traffic inducer(NOSTRIN) in the umbilical vessels of the patients with pre-eclampsia(PE) and discuss its role in the pathogenesis of PE.Methods:Immunohistochemistry was used to detect the expression of NOSTRIN in umbilical vessels and HPIAS(High Resolution Pathological Image Analysis System)-1000 was used to determine the quantity of NOSTRIN;The NOSTRIN mRNA in umbilical tissues was determined by RT-PCR analysis.Results;Immunohistochemistry showed that NOSTRIN expressed in endothelial cells of umbilical vessel in women with PE and normal group;RTPCR showed that the expression level of NOSTRIN was significantly higher in women with PE than in the normal group(P<0.01).Conclusion: It was concluded that the level of NOSTRIN expression increase in umbilical vessel of women with PE,indicating that this maybe involved in the pathogenesis of PE.

  • 【文献出处】 中国优生与遗传杂志 ,Chinese Journal of Birth Health & Heredity , 编辑部邮箱 ,2006年08期
  • 【分类号】R714.246
  • 【下载频次】44
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