【摘要】 目的优选麦冬多糖的提取工艺。方法以麦冬多糖得率作为考察指标,采用正交设计法对麦冬多糖的提取工艺进行优选。苯酚-浓硫酸比色法测定麦冬多糖含量。结果提取工艺以8倍量水,加热30 min,提取2次,醇沉溶度为80%时,麦冬多糖的含量较高。麦冬多糖在0．020 32～0．10008 mg范围内线性关系良好(r=0．999 8),平均回收率为102．41%,RSD为1．78%(n=5)。结论此优选条件麦冬多糖得率较高。更多还原

【Abstract】 Objective To optimize the extraction process and to establish a method for the content determination of the polysaccharides in Ophiopogonjaponicus.Methods With extracts rate of polysaccharide as the index,an orthogonal de- sign test was adopted to optimize the extraction process.And phenol-sulphuric acid colorimetric method was used to de- termine the content of polysaccharides in Ophiopogonjaponicus.Results The optimum extract conditions were as follows: refluxing twice with eight times amount of water,thirty minutes each time,and precipitation with 80 % alcohol.A good linearity of polysaccharides was in range of 0.02032～0.10008 mg(r=0.9998),and the average recovery rate was 102.41%,RSD was 1.78 %.Conclusion The polysaccharide can be fully extracted under these conditions.更多还原