【摘要】 目的建立心可舒片中葛根素的含量测定方法。方法RP-HPLC法。色谱柱：Zorbax Eclipse XDB- C₁₈（4．6mm×150mm,5μm）；流动相：甲醇-水-磷酸（23∶77∶0．5）；检测波长：250nm；流速：1．0mL·min^-1；柱温：25℃。结果葛根素在27．1～271．0 ng范围内线性关系良好,回归方程Y=4．664×10³X（r=0．999 8）,平均加样回收率为100．5%,RSD为1．8%（n=5）。结论本方法操作简便、准确快速、重现性好,可用于心可舒片的质量控制。更多还原

【Abstract】 Objective To establish a RP-HPLC method for determining the content of puerarin in Xinkeshu Tablets. Method The analysis was carried on a column of Zorbax Eclipse XDB-C₁₈（4.6 mm×150mm,5μm）at 25℃,with methanol-water-phosphoric acid（23:77:0.5）as mobile phase.The flow rate was 1.0 mL·min^-1,and the detection wavelength was 250 nm.Result A good linearity was found in the range of 27.1～271.0 ng.The average recovery rate was 100.5 %,and RSD was 1.8%（n=5）.Conclusion The method is simple,rapid,accurate,reproducible,and can be used to control the quality of Xinkeshu Tablets.更多还原