【摘要】 目的建立简单差示蛋白法,获取两种海蛇的特征蛋白用作专属鉴别。方法采用SDS-PAGE与West-ern-blot结合分析并取差示蛋白直接测序。结果确认1号海蛇的特征蛋白为14.6kD及8.7kD两个组分,2号海蛇的特征蛋白为16.7kD及10.2kD两个组分,并完成14.6kD及10.2kD氨基酸测序。结论采用一维电泳结合抗原分析,方法简单,可以获取差示蛋白用于中药材基原鉴别。更多还原

【Abstract】 Objective To identify the specific proteins in two hydrophis (l#and 2#) with a simple differential proteins display method. Method By means of SDS-PAGE and Western blot, combined with analysis, the specific proteins in two hydrophis were obtained and been sequencing.Result The specific proteins of l#are 14.6kD and 8.7kD components and of 2#are 16.7kD. 10.2 kD components. The amino acid sequences of 14.6 kD and 10.2 kD components were assayed respectively. Conclusion Differential proteins can be obtained and used to identify the Chinese traditional medicinal crops by means of one-dimensional electrophoresis combined with antigen analysis .更多还原