【摘要】 目的建立测定莱葛颗粒中葛根素含量的反相高效液相色谱法。方法采用EconosphereC18柱(4.6mm×250mm,5μm);流动相为甲醇-1%醋酸水溶液(25∶75);流速0.8mL/min;检测波长250nm;柱温40℃。结果葛根素在0.2～1.0μg范围内与峰面积具有良好的线性关系,r=0.9997;加样回收率为100.56%,RSD为1.21%(n=5)。结论该方法简便、准确,灵敏度高,重现性好,可作为莱葛颗粒的质量控制方法之一。更多还原

【Abstract】 Objective To establish a RP- HPLC method for quantitative determination of puerarin in Laige Granules. Methods The separation was performed on Econosphere C18 column (4.6 mm× 250 mm,5 μ m) with mobile phase of methanol and 1 % acetic acid solution (25 ∶ 75) at a flow rate of 0.8 mL/min. The UV detection wavelength was 250 nm and the column temperature was 40 ℃ . Results The linear range of puerarin was 0.2～ 1.0 μ g, r = 0.999 7. The mean recovery was 100.56 % (RSD=1.21 % , n = 5 ). Conclusion This method is simple, accurate, sensitive and with good reproducibility .更多还原