【摘要】 建立了一种高效液相色谱法直接测定当归中阿魏酸的含量。色谱柱为Shim-pack CLC-ODS(6.0 mm i.d×150 mm,5μm),流动相为甲醇—1.0%冰醋酸水溶液(体积比为25∶75),流速为1.0 mL/min,检测波长为313 nm。研究结果表明:阿魏酸的线性范围为1.0~10.0 mg/L,回归方程为A=13.352 8C+0.013 6(r=0.998 5),最小检测限为10 ng,加样平均回收率为94.67%,相对标准偏差(RSD)为1.57%。方法操作简便、快速、灵敏、准确。更多还原

【Abstract】 A high performance liquid chromatographic(HPLC) method has been developed for determination of ferulic acid in radix angelicae sinensis.The chromatographic conditions were as follows: Shim–pack CLC-ODS(6.0mm i.d ×150mm column,5 μm) with methanol and 1.0% aqueous acetic acid(25∶75,volume ratio) as mobile phase at a flow rate of 1.0 mL/min and UV detector at 313 nm.The calibration curve was linear within the range of 1.0-10 mg/L(r=(0.998 5)) and the limit of detection was 10 ng for ferulic acid.The average recovery of the method was 94.67%,SRD was 1.57%.The rapid,and accurate method is particularly suitable for the determination of ferulic acid in radix angelicae sinensis.更多还原