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RP-HPLC法测定垂阴茶糖浆中岩白菜素的含量

Determination the Content of Bergenin in Chuiyincha Syrups by RP-HPLC

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【作者】 张文忠蒋平林芳范耀

【Author】 Zhang Wenzhong,Jiang Ping, Lin Fang,Fan Yao (Department of Pharmacy, Affiliated Tongji Hospital,Tongji Medical College,Huazhong University of Science and Technology, Wuhan 43003 ,P. R. China)

【机构】 华中科技大学同济医学院附属同济医院药学部华中科技大学同济医学院附属同济医院药学部 武汉 430030武汉 430030

【摘要】 目的:测定垂阴茶糖浆中岩白菜素的含量。方法:采用反相高效液相色谱法,色谱柱:VP-ODS(150 mm×4.6 mm,5μm),流动相:甲醇-水(15:85);检测波长:275 nm;流速:1.0 ml·min-1;柱温:室温。结果:岩白菜素的线性范围0.25~2.52μg,r=0.999 9,平均回收率为98.76%,RSD%=1.3(n=6)。结论:该法适用于测定垂阴茶糖策中岩白菜素的含量。

【Abstract】 Objective:To set up a RP-HPLC method for the determination of content of Bergenin in Chuiyincha syrups. Method: The VP-ODS column(150 mm×4.6 mm,5μm) was used. The mobile phase consisted of methanol-water (15:85), and the detection wavelength was at 275nm. Result:The linear range of Bergenin was 0.25 - 2.52μg(r = 0.999 9). The average recovery was 98.8%,RSD = 1.3% (n = 6). Conclusion:The method can be used for the determination the content of Bergenin in Chuiyincha syrup.

  • 【分类号】R286.0
  • 【下载频次】33
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