【摘要】 目的:建立HPLC法测定血清中盐酸左氧氟沙星浓度。方法:采用三氯乙酸提取血样,以盐酸洛美沙星为内标,色谱柱:shim-pack C₁₈柱（250 mm×4．6 mm,5μm）,流动相为乙腈:50 mmol·L^-1柠檬酸:1 mol·L^-1醋酸胺（16:83:1）,流速1．0 ml·min^-1,检测波长295 nm,灵敏度0．01AUFS。结果:本法在0．1～10μg·ml^-1之间线性良好（r=0．999 9）,日间和日内RSD小于10％,最低检测浓度为0．1μg·ml^-1。结论:本法快速、准确、重现性好,可为临床血药浓度监测和药物动力学提供依据。更多还原

【Abstract】 To establish a RP-HPLC method for determination the content of levofloxacin hydrochloride in human ser-um. Method: The Shim-pack C₁₈ column（250 mm×4.6 mm, 5μm） was used, mobile phase consisted of acetonitrile-50 mmol·L^-1 citrate -1 mol·L^-1 ammonium acetate （16:83:1）. Flow rate was 1.0 ml·min^-1,detection wavelength was 295 nm. Result: The linear range was 0.1-10μg·ml^-1; Intra-day and inter-day RSD were lower than 10% , the lowest detectable concentration was 0.1μg·ml^-1. Conclusion: The method is rapid, accurate and reproducible. It is suitable for therapeuic drug monitoring.更多还原