【摘要】 目的:探讨夏枯草体外抗淋巴瘤的作用,为临床应用夏枯草治疗淋巴瘤提供实验依据。方法:1.用MTT法测定夏枯草注射液对Raji细胞的生长抑制率,计算出IC50值。同时,用MTT法绘制两种浓度夏枯草注射液(50mg/m l,100 mg/m l)作用于Raji细胞的生长曲线。2.用倒置显微镜、姬姆萨染色法、MTT染色法光镜下观察细胞形态,用免疫细胞化学法检测夏枯草注射液对凋亡相关基因bc l-2、bax蛋白表达的影响,并用图文分析系统进行定量分析。结果:1.夏苦草注射液对Raji细胞生长具有明显的抑制作用,IC50(实际夏枯草浓度)为0.118 mg/m l,且在一定的剂量范围内夏枯草对Raji细胞的抑制作用具有明显的剂量依赖性,相关系数为0.97。2.夏枯草注射液(50mg/m l)作用于Raji细胞后,倒置显微镜,姬姆萨染色,MTT染色光镜下观察,均可见典型的凋亡细胞的形态学特征。免疫细胞化学结果显示,50 mg/m l夏枯草注射液作用于Raji细胞48 h后,bc l-2蛋白表达增强,而bax表示减弱,与对照组相比有显著性差异(P<0.01)。结论:夏枯草可明显抑制Raji细胞增殖,可望成为新的抗淋巴瘤药物,诱导Raji细胞凋亡可能是其发挥抗肿瘤作用的机制之一。更多还原

【Abstract】 Objective: To investigate the anti-lymphoma effect of Prunella vulgaris L.in order to offer exprimental data for the treatment of lymphoma with Prunella vulgaris L.in clinic.Methods: Effect of Prunella vulgaris L.injection on inhibition ratio of cell growth of Raji cells and IC₅₀ were tested by MTT assay.The growth curve line of Raji cells was drawn also by MTT assay.The cellular morphology was observed by invert microscope,Giemas staining and MTT assay.The expression of apoptosis related protein bcl-2,bax was measured by immunocytochemistry and the quantitative analysis was made with figure analysis system.Results: 1.Prunella vulgaris L.could obviously suppress the cell proliferation of Raji cells in a concentration-dependent manner（r=0.97）.The IC₅₀ was 0.118 mg/ml.2.After Raji cells was reacted with injection of Prunella vulgaris L.（50 mg/ml）,the morphlogical of apoptosis were observed by invert microscope,Giemsa staining and MTT assay.Results: The results of immunocytochemistry showed that after Raji cells were treated by the injection of Prunella vulgaris L.（50 mg/ml）for 48 hours,the expression of bcl-1 was up-regulated,and the expression of bax was down-regulated.The differences between process group and control group were significant（P<0.01）.Conclusion: Prunella vulgaris L.can suppress the proliferation of Raji cells and may be a new anti-lymphoma drug.Inducing the apoptosis of Raji cells maybe one of anti-lymphoma mechanisms.更多还原