【摘要】 目的:研究川芎嗪对成体大鼠局灶性脑缺血后皮质和纹状体缺血半暗带细胞增殖的作用,初步探讨川芎嗪对脑缺血损伤修复的可能途径。方法:线栓法制作大鼠左侧大脑中动脉阻塞模型(MCAO),术后2 h腹腔注射川芎嗪(80 mg/kg,1次/d)、4 h腹腔注射B rdU(50 mg/kg,1次/d)。分别于MCAO后7、14、21 d采用免疫组织化学染色观察皮质和纹状体缺血半暗带B rdU标记细胞数量的变化。结果:缺血模型组于MCAO 7 d,在皮质和纹状体缺血半暗带分布有B rdU标记细胞,于14 d明显增加,21 d达峰值。川芎嗪组在皮质和纹状体缺血半暗带亦可观察到B rdU标记细胞,且随缺血时间延长而增加、密集,21 d达峰值;7、14、21 d B rdU标记细胞数量均明显高于相应时间点的缺血模型组(P<0.01)。结论:川芎嗪可促进大鼠脑缺血后皮质和纹状体缺血半暗带神经细胞增殖,以修复、替代损伤的神经细胞,对脑功能自身恢复起重要作用。更多还原

【Abstract】 Objective: To explore the effect of Ligustrazine on cells proliferation in cortex and striatum after focal cerebral ischemia in adult rats.Methods: Focal cerebral ischemia was induced by left middle cerebral artery occlusion(MCAO) with suture method.Two hours later,injection of Ligustrazine(80 mg/kg,1 time/d) was performed peritoneally.Four hours after the ischemia,5-bromodeoxyuridine(BrdU)(50 mg/kg,1 time/d) was injected peritoneally.At 7d,14d and 21d after ischemia,BrdU-Labeled cells in the cortex and striatum were observed by immunohistochemical staining.Results: In ischemia model group,at 7 day,BrdU-labeled cells were observed in the ipsilateral cortex and striatum.With the prolongation of ischemia,the number of BrdU-labeled cells increased,reached the peak at 21d.In Ligustrazine group,BrdU-labeded cells were observed with an intense distribution in ischemic penumbra of the cortex and striatum.With the prolongation of ischemia,the number of BrdU-labeled cells increased significantly at 14d,and reached the peak at 21d.The numbers of BrdU-labeled cells at 7d,14d and 21d were more than those in ischemia model group respectively.Conclusion: Ligustrazine increase the proliferated cells in the cortex and striatum after focal cerebral ischemia in adult rats. The results suggest that it may be useful for promoting self-repair after ischemia.更多还原