【摘要】 目的:探索银杏叶的适宜采收树龄、采收性别以及采收的季节。方法:用乙醇沉淀法从不同树龄、不同性别和不同采收期的12种银杏叶中提取出多糖,并进行差异显著性分析,采用HPLC法测定得率最高的一种多糖的含量。结果:银杏叶多糖的平均得率为4.29%,树龄、性别和采收期的组间、组内均有显著或极显著差异,其中10年生雌树、九月下旬采收的银杏叶多糖的得率最高,为5.44%,其多糖含量为61.5%(RSD=2.5%)。结论:不同树龄、不同性别和不同采收季节的银杏叶中多糖含量有差异,这为银杏资源的合理应用提供了依据。更多还原

【Abstract】 Objective: To determin the suitable tree’s age,tree’s sex and gathering seasons for Ginkgo biloba leaves.Methods: The twelve different polysaccharides were obtained by extracting and precipitating from Ginkgo biloba leaves and to see if there were differences among them.The concentration of Ginkgo biloba leaf polysaccharides with the highest gain ratio will be determined by HPLC.Result: The average gain ratio of Ginkgo biloba leaf polysaccharides was 4.29%,among them the gain ratio of 10-years old female Ginkgo biloba leaf collected in the last ten days of September was the highests,its polysaccharides concentration was 61.5% with RSD=2.5%(n=6).Conclusion: The gain ratios were different in different Ginkgo biloba leaves and the changing rules provide scientific basis for the GAP of medicinal plants.更多还原