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贵州天麻种质资源的RAPD分析
RAPD Analysis on the Germplasm Resources of Gastrodia elata in Guizhou
【摘要】 对贵州省境内药用植物天麻的野生和栽培15个样品的基因组DNA遗传多态性的RAPD分析。从40个随机引物中筛选出12个有效引物,共检测到93个位点,其中66个显多态性,总的多态位点百分率(PPB)为70.97%。经NTSYSpc,ver.2.2软件进行UPGMA聚类分析,结果表明野生与栽培天麻之间的遗传变异较大,说明地理分布和生长环境的差异是导致天麻形成丰富的遗传多态性的一个重要的原因。
【Abstract】 Genetic polymorphisms of genomic DNA of 15 samples from wild and culturaled Gastrodia elata Blume in Guizhou were analyzed by RAPD method.12 effective primers are screened from 40 primers amplified a total number of 93 loci,among which 66 are polymorphic and the percentage of polymorphic loci(PPB) is 70.97%.UPGMA dendrogram analyzed by NTSYSpc,ver.2.2 shows that Gastrodia elata have apparent genetic variance.Geological distribution and growing environment were significant factors for the polymorphism.
【基金】 科技部2004BA721A33;贵州省科技黔科合社字(2001)1125号;黔科合中药专字[2003]51号课题
- 【文献出处】 中药材 ,Journal of Chinese Medicinal Materials , 编辑部邮箱 ,2006年09期
- 【分类号】R282.2
- 【被引频次】31
- 【下载频次】344