【摘要】 目的:观察甘草抗病毒有效部位(GD4)抑制呼吸道合胞病毒(RSV)作用。方法:采用煎煮法、二氯甲烷萃取法和柱层析法提取分离得到甘草抗病毒有效部位(GD4)。采用细胞病变抑制实验,观察GD4在Hela细胞中对RSV的抑制作用。结果:GD4半数中毒浓度(TC50)为0.23 mg/m l,抑制RSV的半数有效浓度(EC50)为28.73μg/m l,治疗指数(TI)为8.0;GD4对RSV的抑制作用存在量效关系;在感染后至少12 h以内给药,GD4能够有效地抑制RSV的复制。GD4不含有甘草酸。结论:甘草抗病毒有效部位(GD4)在Hela细胞中对呼吸道合胞病毒有明显的抑制作用。更多还原

【Abstract】 Objective: To study the effect on anti-respiratory syncytial virus of an active compound GD4 from Glycyrrhiza in vitro.Methods: GD4 was extracted from Glycyrrhiza by hot water extraction,dichloromethane extraction and column chromatography.The CPE inhibition assay was used to test the antiviral activity of GD4 on RSV in Hela cells.Results: GD4 was effective antiviral agent for RSV in a concentration-dependent manner and its median toxic concentration(TC₅₀) and median effeicacious concentration(EC₅₀) and treatment index（TI） was 0.23 mg/ml,28.73 μg/ml and 8.0.120 μg/ml GD4 exhibited inhibitory effect when added into cell culture for every 2h within 12h post-infection.GD4 didn’t contain glycyrrhizic acid.Conclusion: The active compound of Glycyrrhiza（GD4）was an evident inhibitory effect on RSV.更多还原