【摘要】 目的:研究不同生长阶段麦冬根部的内生真菌。方法:采集不同生长阶段的麦冬根段,切片染色法观察麦冬根内真菌显微结构及真菌侵染的特征,分析麦冬内生真菌与麦冬发育的关系。结果:麦冬根内含有丰富的内生真菌,苗期根内逐渐出现菌丝并形成大量菌丝团,块根发生初期菌丝连续分枝形成丛枝,块根生长和块根膨大期内存在大量泡囊。结论:麦冬根内的真菌为丛枝菌根真菌,不同生长阶段,根内出现内生真菌的菌丝、丛枝、泡囊,显示内生菌可能与麦冬生长、活性成分的合成有关。更多还原

【Abstract】 Objective: To study on the relationship between the endophyte and the life cycle of Ophiopogon japonicus in Zhejiang.Methods: Sample roots of Ophiopogon japonicus at different growth stages were thoroughly washed and cut into small fragments,then cleared(removing cytoplasmic contents from cells) using hot 10% KOH and stained with acid fuchsin(alternative stain).The hyphae,the arbuscular and the vesicular of endophyte were examined.Results: The hyphae appeared and grew in the seedling stage,the hyphae grew into arbuscular in the root tuber generating stage and vesicular in the stage of root tuber expanding period.Conclusion: The endophytes in Ophiopogon japonicus appeared in forms of hyphae,arbuscular and vesicular at different growth stages to meet the needs of Ophiopogon japonicus developing.更多还原