【摘要】 通过采用热水提取、乙醇沉淀获得沙棘叶水溶性粗多糖,再经酸性乙醇分级,将沙棘叶水溶性粗多糖分为三个级分SJ1、SJ2和SJ3,将对自由基有初步清除作用的SJ2脱蛋白后经DEAE-SephdexA-25进行分离纯化得SJ22。SJ22经纸层析、醋酸纤维薄膜电泳和SephroseCL-4B柱层析纯度鉴定表明:SJ22为分子量均一的纯多糖。分子量为7万。纸层析显示SJ22单糖组成为Xy1、Ara、G lc、Gal、GalA。抗衰老活性研究结果表明,SJ22能有效清除.OH、O2-.自由基,对脂质自由基.R效果不明显。更多还原

【Abstract】 The crude polysaccharide was extracted from Leave in Hippohae rhamnoides L.with hot water,and precipitated by ethanol.The crude polysaccharide has been fractionated by acidic ethanol.Three fractions（SJ₁、SJ₂、SJ₃） were got respectively.SJ₂ deproteinizationed by the combined methods of enzyme and Seveage,purified by DEAE-Sephadex A-25 gel filtration.PC analysis indicated that SJ₂₂ is composed of Xyl、Ara、Glc、Gal、GaL A.The identification of purify by Sepharose CL-4B,paper chromatography and cellulous acetate electrophoresis showed it was homogeneous.Typical absorption of polysaccharides was shown in its IR spectrum.It contained α-glucosidic bonds by IR analysis.It had typical absorption of protein by UV scaning.SJ₂₂ is first isolated from Leave in Hippohae rhamnoides L.Scavenging free radical experiment showed that SJ₂₂ was effective in scavenging superoxideradical and hydroxylradical,but only a little effective in scavenging lipid radical.更多还原