【摘要】 目的:建立河北道地药材北柴胡的HPLC-UV指纹图谱分析方法,并与不同产地柴胡药材指纹特征相比较,为科学评价与有效控制柴胡质量提供新方法。方法:采用HPLC-UV法测定了道地北柴胡等21个不同来源的柴胡样品。色谱条件:C18柱,乙腈-水为流动相进行梯度洗脱,检测波长203 nm,流速1.0 m l/m in,柱温30℃。结果:建立了北柴胡HPLC-UV指纹图谱共有模式,并对不同产地柴胡药材进行了相似度比较。结论:色谱指纹图谱分析法能简便、快速鉴别和区分不同来源的柴胡药材,为全面控制柴胡药材的质量提供了依据。更多还原

【Abstract】 Objective: To establish a HPLC fingerprint analysis method for identification of Bupleurum chinense of Hebei Province and compare the fingerprints of Radix Bupleuri collected from different habitats so as to establish a sensitive and specific method for controlling the quality of Radix Bupleuri.Methods: The HPLC-UV fingerprints of Radix Bupleuri from different habitats were obtained from Waters 1525 instruments.The HPLC separation was performed on a C₁₈ analytical column gradient eluted with a mixture consisting of acetonitrile and water at a flow rate of 1.0 ml/min with UV detector at 203 nm.The temperature of column was 30℃.Results: The mutual mode of HPLC-UV fingerprints was set up,and the similar degrees to the crude drugs of different habitats were compared.Conclusion: It is simple and quick to differ Bupleurum chinense from different habitats with the method that can be used as a quality control item for Radix Bupleuri.更多还原