【摘要】 目的:观察蒺藜总皂苷（gross saponins from Tribulus terrestris L,GSTT）对缺氧复氧心肌细胞炎症分子肿瘤坏死因子-α（TNF-α）,白介素1β（IL-1β）及细胞间黏附分子-1（ICAM-1）表达的影响。方法:利用原代培养的SD乳鼠心肌细胞建立心肌缺氧复氧模型,分为正常组、模型组和GSTT高、低浓度(50,10mg·L^-1)组。用放免法检测TNF-α和IL-1β,细胞ELISA检测ICAM-1表达。结果:与模型组比较,GSTT高、低浓度(50,10mg·L^-1)组TNF-α和IL-1β含量显著降低（P＜0．01或P＜0．05）;GSTT 50 mg·L^-1组ICAM-1蛋白表达量显著降低（P＜0．05）。结论:GSTT对缺氧复氧损伤心肌细胞有保护作用,其机制与抑制炎性因子TNF-α和IL-1β释放,降低ICAM-1蛋白表达有关。更多还原

【Abstract】 Objective:To investigate the effects of gross saponins from Tribulus terrestris L （GSTT） on expression of inflammatory molecules of TNF-α,IL-1βand ICAM-1 in cardiocytes with hy- poxia/regeneration injury.Methods:A model of cardiocytic hypoxia/reoxygenation was induced by pri- mary cultures of cardiocytes of SD neonatal rats in vivo.Subsequently,the cardiocytes were incubated with the GSTT 0,10 or 50 mg·L^-1,respectively.Additional healthy eardiocytes of SD rats were used as normal control.TNF-αand IL-1βconcentrations were measured by radioimmunoassay,and ICAM-1 were detected by ELISA.Results:The GSTT (10 and 50 mg·L^-1) led to significant reductions of TNF-αand IL-1βlevels （P＜0.01,P＜0.05）.The ICAM-1 levels were significantly decreased in the incubation of the cardioeytes with the GSTT 50 mg·L^-1 （P＜0.05）.Conclusion:The GSTT protected the eardiocytes from hypoxia/regeneratiou injury by inhibiting the release of TNF-αand IL-1βas well as decreasing the expression of ICAM-1.更多还原