【摘要】 目的:建立高效液相色谱法检测泛酸钠原料中的有关物质。方法:氨基硅烷键合相色谱柱（300 mm×4．6 mm,5μm）;流动相:0．05 mol·L^-1磷酸二氢钾溶液-乙腈（30:70）,用10%磷酸溶液调节pH为5．8;检测波长: 214 nm;流速:1．5 mL·min^-1。结果:杂质峰与主峰能够得到有效的分离,最低检出浓度为10 ng·mL^-1,3批泛酸钠样品经检测有关物质分别为0．10%,0．08%,0．12%。结论:该法可用于检查泛酸钠原料中的有关物质,简便、准确。更多还原

【Abstract】 Objective:To establish a HPLC method for determination of the related substances in D-sodium pantothenate.Methods:The HPLC condition was as follows:a NH₂ column（300mm×4.6mm,5μm）,the mobile phase consisted of 0.05 mol.L^-1 monobasic potassium phosphate solution-acetonitrile （30:70）（pH 5.8,adjusted with 10% phosphoric acid） with a flow rate of 1.5 mL·min^-1 and the UV detection at 214 nm.Results:The major components could be satisfactorily separated from the related substances.The detection limit was 10 ng·mL^-1.The impurity rate of three batches of D-sodium pantothenate samples was 0.10%,0.08% and 0.12%,respectively.Conclusion:The simple and accurate HPLC method could be used in the quality control of D-Sodium pantothenate.更多还原