Objective To study the effects of Eclipta prostrata L (Mohanlian) ethanol extract, which activates tyrosinase in vitro, on melanogenesis and expression of its related genes(TYR, TRP-1/2 mRNA) in cultured B16 melanoma cells. Methods Using brownish guinea pigs as experimental models, cells containing melanin granules and DOPA-positive cells in 100 basal cells were counted by Schmorl and DOPA-oxidase stain methods respectively. The cells proliferation, melanogenesis and tyrosinase activity in cultured B16 cell...
【基金】
辽宁省自然基金课题资助(972290)
【更新日期】
2006-05-08
【分类号】
R285.5
【正文快照】
中草药墨旱莲在中医治疗白癜风方剂中常被采用,近年来有报道离体实验中墨旱莲有促进酪氨酸酶(tyrosinase,TY R)活性的作用犤1,2犦,激活TY R活性的动力学研究亦有报道犤3犦。本实验以棕色豚鼠为动物模型,采用Schm orl法染色和多巴(D O PA)-氧化酶染色,分别计数黑素细胞颗粒和每1