【摘要】 目的:探讨商陆抗病毒蛋白(PAP)对丙型肝炎病毒(HCV)感染细胞模型中HCV复制的影响。方法:用不同浓度PAP处理HCV感染细胞模型(HCV-HepG2),以荧光定量PCR法检测培养上清液中及细胞内HCV RNA,以不同浓度的α-2b干扰素(IFNα-2b)处理同样的细胞模型作为对照。结果:经PAP处理后各模型组细胞内HCVRNA含量于48小时、96小时和144小时进行比较,差异有非常显著性意义(P<0·01);培养上清液中HCV RNA含量在48小时时,各组间比较,差异无显著性意义(P>0·05),但在96小时和144小时时,各组间比较差异有非常显著性意义(P<0·01)。PAP对HCV的抑制作用随PAP浓度的增加而增强,以100μg/ml浓度的抑制作用最强。用IFN-α2b处理HCV-HepG2模型亦得出类似的结果。两者均以第48小时的抑制率最高。但在96小时、144小时时PAP对细胞内HCV的抑制作用明显强于IFN(P<0·05,P<0·01)。结论:PAP对HCV感染细胞模型中的HCV复制有明显地抑制作用,且作用强于IFN-α2b。PAP对细胞无明显毒性。更多还原

【Abstract】 Objective:To investigate the influence of Pokeweed antiviral protein （PAP） on hepatitis C virus （HCV） replication in an infection cell model（HepG₂）.Methods:Using fluorescent quantitative PCR to test the copies of HCV RNA in the infected cells treated with different concentration of PAP, as well as the supernatant. Interferon α-2b （IFNα-2b） treatment was used as a control.Results: HCV RNA copies in the infected cells were significantly different among different groups at 48, 96,144 hours post PAP treatment（P<0.01）.HCV RNA copies in the supernatant were also significantly different at 96, 144 hours post PAP treatment （P<0.01）,but not at 48 hours（P>0.05）. The inhibition efficiency of APA on HCV replication increased when using higher concentration, and reached up to the peak at 100μg/ml. Similar results were obtaind from the IFNα-2b treated groups. The inhibition efficiency of both PAP and IFNα-2b were the highest at 48 hours post-treatment, however, PAP was more efficient than IFNα-2b at 96 hours and 144 hours post-treatment （P<0.05,P<0.01）. Conclusion:PAP can efficiently inhibit HCV replication in the HepG₂ cell model, and its efficiency is higher than IFNα-2b. PAP is not obviously toxic to the HepG₂ cells.更多还原