【摘要】 目的以苯磺酸氨氯地平为模型药,制备传递体并对其体外分析方法进行确立。方法采用薄膜-超声法制备苯磺酸氨氯地平传递体(AM-T),用激光粒度分析仪分析其大小与分布,采用紫外分光光度法(UV)和反相高效液相色谱法(RP-HPLC)测定药物的回收率及含量等。结果苯磺酸氨氯地平传递体的平均粒径在60nm左右。UV法和HPLC法所得苯磺酸氨氯地平检测浓度线性范围均为1～50μg/mL;平均回收率分别为(100.12±0.54)%和(99.88±0.57)%;含量分别为(101.40±1.75)%和(100.60±0.86)%。结论该传递体的制备方法可行,建立的体外分析方法稳定、可靠。更多还原

【Abstract】 [Objective] To prepare transfersomes with Amlodipine Besylate and establish its analysis methods in vitro.[Methods] Amlodipine Besylate transfersomes(AM-T)was prepared by film-ultrasonic technique.The recovery and concentration of AM-T was determined by ultraviolet spectrophotometry(UV)and reversed-phase high performance liquid chromatography(RP-HPLC).[Results] The mean particle size of AM-T was well-distributed in 60 nm.The linear detection range of AM-T concentration was 1-50 μg/mL.The average recovery is(100.12±0.54)% and(99.88±0.57)%,and the concentration is(101.4±1.75)% and(100.6±0.86)% determined by UV method and RP-HPLC method respectively.[Conclusion] The transfersomes are feasible in preparation techniques and the analysis methods in vitro is stable and reliable.更多还原