【摘要】 目的探讨18种人肿瘤细胞对化疗药物的敏感性与基因表达的关系。方法采用MTT法检测细胞株对阿霉素(ADM)、顺铂(DDP)、丝裂霉素(MMC)、5-氟尿嘧啶(5-Fu)和卡氮芥(BCNU)的敏感性。流式细胞术检测P-糖蛋白(P-gp)、谷胱甘肽-S-转移酶-π(GST-π)、肺耐药蛋白(LRP)、多药耐药相关蛋白(MRP)和甲基鸟嘌呤甲基转移酶(MGMT)等耐药相关基因和Fas、bcl-2、p53和p16等凋亡调控基因的表达。结果不同细胞株对同一药物敏感性有较大差异。相关分析表明,细胞株对DDP的敏感性与p53表达呈负相关(P=0.041),对其余药物的敏感性与所检基因的表达水平无关(P>0.05)。结论细胞株对DDP的敏感性与p53表达有关。更多还原

【Abstract】 [Objective] To explore the relationship of chemotherapy sensitivity and gene expression in 18 human cancer cell lines. [Methods] MTT method was used to detect the sensitivity on Adriamycin(ADM), Cisplatinum(DDP), Mitomycin C(MMC), Fluorouracilum(5-Fu), Carmustine (BCNU) in cancer cell lines. Multidrug resistance genes including P-glycoprotein (P-gp), Glutsthione-s-transferases-π (GST-π), Lung resistance protein (LRP), Multidrug resistance related protein (MRP), MGMT and apoptosis regulation genes Fas, bcl-2, p53 and p16 were examined by flow cytometry (FCM). [Results] The chemotherapy sensitivity was obviously divergent in different cancer cell lines. The correlation analysis revealed that there were negative correlations between the sensitivity on DDP in cell lines and the expression of p53 protein, the P value was 0.041. There were no correlations among the sensitivity on other drugs and expression of other genes (P >0.05). [Conclusions] The sensitivity on DDP had correlation with the the expression of p53 in cancer cell lines.更多还原