【摘要】 目的观察实验感染HEV(HepatitisEVirus)IV型后恒河猴HEVRNA及抗HEV-IgM(IgG)的动态变化,探索HEVIV型感染的规律。方法用逆转录PCR检测实验动物接种前后血清及粪便HEVR-NA,用酶联免疫法(ELISA)检测血清抗HEV-IgM及抗HEV-IgG,并同步观察其血生化及肝脏组织学的变化。结果感染后的恒河猴均发生了典型的急性肝炎,血清HEVRNA首先呈现阳性,相继出现谷丙转氨酶(ALT)升高、抗HEV-IgM阳性及抗HEV-IgG阳性。结论中国恒河猴是HEVIV型感染的较好的动物模型,检验资料为阐明HEVIV型感染的规律提供了证据。更多还原

【Abstract】 [Objective] To observe the dynamic changes of HEVRNA and Anti-HEV IgM (IgG) in China Rhesus infected by HEV-IV. To study the rules of infection of IEV-IV. [Method] A specific RT-PCR assay was used for the detection of HEV-RNA in sera and stool suspensions of experimentally infected China Rhesus; ELISA was used for the detection of anti-HEV IgM and IgG in sera. The changes of sera biochemistry and liver histopathology were observed synchronizedly. [Result] The two experimental animals got typical acute hepatitis profile. [Conclusion] China Rhesus was a good experiental animal model of HEV-IV. The result showed solid HEV infection evidence of typical hepatitis after the infection.更多还原