【摘要】 目的利用基因工程原理合成携带血红素加氧酶1(HO1)基因的乳酸乳球菌,通过对正常大鼠灌胃后,观察其减轻失血性休克大鼠肠道炎症反应及肠黏膜屏障的保护效应。方法按随机数字表法将30只健康清洁级雄性SD大鼠分为携带HO1基因的乳酸乳球菌灌胃组(HO组,n=10)、乳酸乳球菌灌胃组(LL组,n=10)及磷酸盐缓冲液灌胃组(PBS组,n=10)。实验前24h灌胃,复制失血性休克模型。液体复苏1h后取材,比较各组动物的死亡率、细菌移位以及肠组织髓过氧化物酶(MPO)活性、HO1、肿瘤坏死因子α(TNFα)和白细胞介素10(IL10)含量,并检查肠组织病理学变化。结果与LL组和PBS组比较,HO组的死亡率、Chiu6级评分、细菌移位发生率均明显降低(P均<0.05);HO1含量和IL10灰度值均明显增加(P均<0.05);与HO组和LL组比较,PBS组肠组织MPO活性明显增加(P<0.05)。结论携带HO1基因的乳酸乳球菌对失血性休克大鼠有较好的肠黏膜屏障保护作用,能明显减轻肠道炎症反应和细菌移位的发生率。更多还原

【Abstract】 Objective To evaluate the effects of gavage with lactococcus lactis (L. Lactis) recombinant heme oxygenase1 (HO1) gene on alleviation of intestinal inflammation and protection of the intestinal mucosa in rats with hemorrhagic shock. Methods A model of rats with hemorrhagic shock was reproduced in 30 healthy SD male rats. The rats were randomly divided into the L. Lactis recombinant HO1 gene group (HO group, n=10), L. Lactis group (LL group, n=10) and phosphate buffer group (PBS group, n=10). These agents were respectively gavaged 24 hours before the experiment. Rats were re-anesthetized 1 hour after fluid resuscitation. The mortality, myeloperoxidase (MPO) activity, bacterial translocation, the pathologic changes, the contents of HO1, tumor necrosis factorα (TNFα) and interleukin10 (IL10) in the intestine were determined and compared. Results Compared with LL group and PBS group, the mortality, Chiu′s grade and the bacterial translocation rate of HO group were significantly decreased (all P<0.05) but the content of HO1 and the level of IL10 in HO group were markedly increased (both P<0.05). Compared with HO group and LL group, the MPO activity of PBS group was obviously increased (P<0.05). Conclusion The recombinant L. Lactis has the effect to deliver HO1, which has protective effect on the intestinal mucosa in lessening the inflammation of the intestine and the incidence of bacterial translocation.更多还原