【摘要】 目的观察休克期大面积切痂对严重烧伤大鼠细胞免疫功能的影响,探索改善烧伤后机体免疫功能紊乱的有效方法。方法将大鼠分成休克期切痂组(A组)、常规切痂组(B组)和正常对照组(C组)。A、B组造成30%TBSAⅢ度烫伤,C组不烫伤。A组伤后第6 h、B组伤后第4 d切痂,并于伤后第1、5、9 d各活杀10只,取材送检,观察其免疫指标的变化。结果(1)A、B组与C组比较:A、B组烫伤大鼠各时相点CD3+T细胞变化不大(P>0.05),但CD4+T细胞、CD4+/CD8+比值明显下降、CD8+T细胞增高(P<0.05或P<0.01),NK细胞活性明显下降(P<0.05或P<0.01),外周血CD25+T淋巴细胞表达及经活化后脾脏CD25+T淋巴细胞表达明显下降(P<0.05或P<0.01)。(2)A组与B组比较:A组CD4+T细胞、CD4+/CD8+比值明显升高、CD8+T细胞降低(P<0.05或P<0.01),NK细胞活性明显升高(P<0.05或P<0.01),外周血CD25+T淋巴细胞表达及经活化后脾脏CD25+T淋巴细胞表达均明显升高(P<0.05或P<0.01)。结论(1)大鼠烫伤后细胞免疫状况发生了明显变化。(2)休克期切痂可以改善烫伤大鼠T淋巴细胞亚群分布,提高NK细胞活性,增加外周血CD25+T淋巴细胞的表达,提高经活化后脾脏CD25+T淋巴细胞数,从而改善烫伤大鼠伤后机体的细胞免疫功能。更多还原

【Abstract】 Objective To investigate the effect of escharectomy during shock stage on cellular immune function in severe scaled rats,illustrate a way to improve immunity function disturbance of burned rats.Methods Wistar rats were subdivided randomly into three groups: group A escharectomy during shock stage,group B routine therapy,group C as control.The rats of group A and group B were made 30% TSBA Ⅲ degree scalding.Rats were given escharectomy at 6 hour postburn in group A and at 4 day postburn in group B.Ten rats were killed in 1,5 and 9 days postburn respectively in group A and B and samples were collected to experiment.Results（1）There was no significant diversity in CD3⁺among three groups（P>0.05）.CD4⁺,CD4⁺/CD8⁺ ratio,NK cells activity,the CD25⁺ expression of T-lymphocyte of peripheral blood,the CD25⁺ expression of the splenic T-lymphocyte in vitro through Con A culture in rats with severe burn were significantly lower while CD8⁺ greatly higher than that in controll group（P<0.01 or P<0.05）.（2） CD4⁺、 CD4⁺/CD8⁺ ratio,NK cells activity the CD25⁺ expression of T-lymphocyte of peripheral blood,the CD25 expression of the splenic T-lymphocyte in vitro through Con A culture in rats with severe burn after escharectomy during burn shock stage were significantly higher while CD8⁺ was greatly lower than that in routine group（P<0.01 or P<0.05）.Con-clusion The immunity was suppressed in the rats with severe burn.Escharectomy during burn shock stagemay be beneficial to improving the T-lymphocyte subsets,NK cell,the CD25⁺ expression of T-lymphocyte of peripheral blood and the splenic T-lymphocyte in vitro through Con A culture.更多还原