【摘要】 从salk库中取得1种PLC4基因突变体,PCR检测表明其T-DNA插入位点比网上的位置有235bp的偏差,在PLC4基因的3’非翻译区,位点的插入造成该基因的超表达。表型分析表明,该突变株的花粉形态与野生型有差异。推测该基因的超表达对花粉发育造成影响。更多还原

【Abstract】 A PLC4 gene mutant was obtained from Salk Institute Genomic Analysis Laboratory.Insertion site of T-DNA differed about 235 bp from that of Salk Laboratory by PCR,T-DNA inserted 3’ UTR.This insertion site resulted in PLC4 overexpression.Mutant pollen appeared morphologically unnormal compared with wide-type.So we presumed that PLC4 overexpression affected pollen development.更多还原