【摘要】 大叶黑桫椤孢子在液氮中长期保存的结果表明:(1)大叶黑桫椤孢子可以保存于液氮中;(2)液氮保存后的孢子萌发率比未用液氮保存的普遍下降,保存90D的用室温慢速化冻的孢子萌发率最高(65.3%),相对保持率最高可达79.3%;(3)采用室温(22￣25℃)慢速化冻的效果优于37℃温水浴快速化冻的。据此认为,液氮超低温长期保存大叶黑桫椤孢子是可行的。更多还原

【Abstract】 The results of the long-term storage of Alsophila gigantea var. gigantea Wall. ex Hook. in the liquidnitrogen (LN) showed that: (1) Spores of A. gigantea var. gigantea could successfully survive the liquidnitrogen. (2) After thawing, the spore germination rate was comparatively lower than that of the control, themaximum germination (65.3%) was obtained from the spores cryopreserved for 90 d upon air thawing at roomtemperature, and the relative maintenance percentage reached 79.3%. (3) Air thawing at room temperature(22~25℃) resulted in a higher percentage of spore germination than upon rapid thawing in 37℃ water. Theresults demonstrated the feasibility of cryopreservation for the long-term storage of spores of A. gigantea var.gigantea.更多还原