【摘要】 脱落酸(abscisic,ABA)预处理可以显著提高铁皮石斛(Dendrobiumcandidum)类原球茎体(protocrom-likebodies,PLBs)的耐脱水性,其中以5μmol/L的ABA预处理24h效果最好。经2h快速脱水后,类原球茎体存活率约为对照的10倍,达到50%左右。预处理期间细胞相容性物质含量的研究表明,ABA预处理过程中可溶性总糖、蔗糖、可溶性蛋白质及脯氨酸含量均无明显变化,而可溶性多糖含量明显增加,作者认为可溶性多糖的积累是类原球茎体耐脱水性提高的原因之一。更多还原

【Abstract】 Effect ofABA on desiccation tolerance in the protocorm-like bodies (PLBs) of D.can- didum was studied. The best result was obtained while ABA precultured at 5 μmol/L for 24 h (Fig. 2). PLBs vitality rate was 10 times of the control after 2 h desiccation (Table 1). Detection of cyto- plasmic osmoticums showed that ABA preculture could not induce the accumulations of soluble sugar, proline, etc (Tables 3,4 and 7).But the con- tent of solublepolysaccharide rose conspicuously (Table 5). So we think that the accumulation of soluble polysaccharide may play an important rolein desiccation tolerance induced by ABA preculture.更多还原