【摘要】 以成熟种子为来源的胚性愈伤组织为受体材料,采用根癌农杆菌介导方法将反义磷酸烯醇式丙酮酸羧化酶(phosphoenolpyruvatecarboxylase,PEPCase)基因导入粳稻品种秀水11,获得一批转基因植株,并通过GUS组织化学染色、PCR检测和Southern杂交分析等方法进行了验证。对T1代的检测结果表明,所转基因已遗传给后代,且大多数株系的分离比符合3:1。T2代测定结果显示,转基因水稻株系的稻米脂肪含量比对照高出0.37±0.12个百分点,其差异大部分达到极显著水平。更多还原

【Abstract】 Geneforphosphoenolpyruvatecarboxy- lase(PEPCase) was incorporated in antisense ori- entation into genome of japonica rice variety Xiushui 11 through Agrobacterium tumefaciens- mediated transformation of mature seeds-derived embryogenic calli, and a total of 95 transgenic plants wereregenerated, which wereconfirmed by histochemical detection of GUS activity (Fig.2), PCR assay (Fig.3) and Southern hybridization analysis (Fig.4). The transgene was inherited by the T1 progeny and Mendelian 3:1 segregation ra- tio was observed in most transgenic lines (Table1). Itwas found that the fat contentofhulled grains ofT2 transgeniclines was0.37±0.12 percenthigher than that of the control, most of which were statis- tically very significant (Table 2).更多还原