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干旱胁迫对小麦幼苗抗氰呼吸和活性氧代谢的影响

Effects of Drought Stress on Cyanide-resistant Respiration and Metabolism of Reactive Oxygen in Wheat Seedling

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【作者】 吴强冯汉青李红玉万东石梁厚果

【Author】 WU Qiang, FENG Han-Qing, LI Hong-Yu*, WAN Dong-Shi, LIANG Hou-GuoSchool of Life Sciences, Lanzhou University, Lanzhou 730000, China

【机构】 兰州大学生命科学学院兰州大学生命科学学院 兰州730000兰州730000

【摘要】 研究了干旱胁迫对抗旱性强弱不同的两种小麦幼苗的抗氰呼吸和活性氧代谢的影响。干旱胁迫导致了两种小麦抗氰呼吸活性及基因转录水平的下降,但抗旱品种在轻度干旱胁迫下表现出一定的适应能力,其抗氰呼吸活性及基因转录水平均高于不抗旱品种。干旱胁迫下,对干旱敏感的小麦幼苗叶片中活性氧含量高于抗旱小麦;3种抗氧化酶的活性低于抗旱小麦的3种抗氧化酶的活性。据此认为,严重的干旱胁迫引起活性氧含量的增加扰动了活性氧与抗氰呼吸之间的应答平衡,但抗氰呼吸可能通过清除活性氧等机制而起了抗旱的作用。

【Abstract】 The changes in cyanide-resistant respi-ration and metabolism of active oxygen species indrought-sensitive and drought-resistant wheat seed-lings under water stress were studied. In general,drought stress decreased the level of cyanide-re-sistant respiration and AOX1 mRNA content inwheat seedling leaves (Figs.1–3, 5). But thedrought-resistant cultivars, which had much higherlevel of cyanide-resistant respiration and AOX1mRNA content than the drought-sensitive ones(Figs.1–3, 5), presented an ability to adapt to milddrought stress. Under drought stress, the drought-sensitive wheat seedling leaves had higher O2·ˉ andH2O2 contents, but lower SOD, POX and CAT ac-tivities than drought-resistant cultivars (Figs.6, 7).We guessed that severe drought stress could breakdown the balance between active oxygen speciesand cyanide-resistant respiration. However, cya-nide-resistant respiration still could participate inthe drought-resistance by lowering the level of ac-tive oxygen species besides by other mechanism.

【基金】 国家自然科学基金项目(Nos.30471133,30170238)资助。~~
  • 【文献出处】 植物生理与分子生物学学报 ,Journal of Plant Physiology and Molecular Biology , 编辑部邮箱 ,2006年02期
  • 【分类号】S512.1
  • 【被引频次】55
  • 【下载频次】1252
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