【摘要】 目的:比较ELISA法与HPLC法检测水中微囊藻毒素含量的异同。方法:利用基于抗MC-LR多克隆抗体建立的间接竞争ELISA和HPLC法分别检测太湖中不同检测点、不同时期采集的大量水样中的MC-LR含量。结果:ELISA法和HPLC法相对误差平均值为8.62%,相关性检验t值为0.9996。在加标浓度为0.5~12.5 ppb时,ELISA法回收率为94.84%~116.51%,HPLC法回收率为86.14%~92.18%。结论:两种方法具有良好的相关性,但ELISA法比HPLC法前处理简单,快速,回收率较好,更适合基层检测部门使用。更多还原

【Abstract】 Objective:Compare the similarities and differences between ELISA and HPLC on detecting the microcystins in water.Methods:Using ELISA which is built on anti-MC-LR antibody and HPLC at one time to detect the MC-LR in Taihu water gathered of deferent period and field.Results:The average of relative errors between ELISA and HPLC is 8.62%,the value of correlation test CHITEST is 0.9996.The recovery rates of ELISA were in 94.84%～116.51% while the concentration of MC-LR which was added was 0.5~12.5 ppb,and the recovery rates of HPLC were in 86.14%～92.18%.Conclusion:The test results on two methods were good correlation,furthermore ELISA were more simpler,faster,and better recovery rates than HPLC.So,ELISA was a suitable method for the basic detection departments.更多还原