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栽培黄瓜与野黄瓜正反杂交的几种同工酶分析

Isozyme analysis of reciprocal interspecific hybrid F1 between Cucumis sativus and its wild relative C. hystrix

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【作者】 罗向东戴亮芳刘强娄群峰钱春桃陈劲枫

【Author】 LUO Xiang-Dong 1, 2DAI Liang-Fang 1LIU Qiang 1LOU Qun-Feng 1QIAN Chun-Tao 1CHEN Jin-Feng* 1 (State Key Laboratory of Crop Genetics and Germplasm Enhancement, College of Horticulture, Nanjing Agricultural University, Nanjing 210095, China) 2 (College of Life Sciences, Jiangxi Normal University, Nanchang 330027, China)

【机构】 作物遗传与种质创新国家重点实验室南京农业大学园艺学院作物遗传与种质创新国家重点实验室南京农业大学园艺学院 南京210095 江西师范大学生命科学学院南昌330027南京210095 江西师范大学生命科学学院南昌330027南京210095

【摘要】 运用天冬氨酸转氨酶(AAT)、苹果酸脱氢酶(MDH)以及酯酶(EST)3种同工酶对栽培黄瓜“长春密刺”Cucumissativuscv.Changchunmici(2n=14)与野黄瓜C.hystrix(2n=24)的正反交种间杂种F1(正交:野黄瓜×栽培黄瓜“长春密刺”,反交:栽培黄瓜“长春密刺”×野黄瓜)及其双亲进行鉴定和比较研究。结果表明:正反交种间杂种F1主要表现为双亲酶带的互补,同时还形成4个杂合带(Aat-1-94、Aat-2-104、Mdh-3-102和Est-5-102)。上述3种酶均能准确地鉴定种间杂种的真实性。研究还发现正反交杂种F1的AAT和MDH的酶谱分别在酶带数目和强弱上表现出一定的差异,进一步证实了野黄瓜与栽培黄瓜杂交存在正反交差异。

【Abstract】 Electrophoresis of aspartate aminotransferase (AAT), malate dehydrogenase (MDH) and esterase (EST) were performed to characterize and compare the reciprocal interspecific hybrid plants between cultivated cucumber (Cucumis sativus cv. Changchunmici, 2n=14, CC) and its wild relative C. hystrix (2n=24, HH), and their parents. The results indicate that the zymograms in the reciprocal F1 were typically expressed by complementary bands from both parents. In addition, four heterodimeric bands (Aat-1-94, Aat-2-104, Mdh-3-102 and Est-5-102) were observed. All the three isozymes investigated could identify the interspecific hybrid plants. The results also reveal the differences of the reciprocal F1s at the number and intensity of the bands in the zymograms of AAT and MDH, which further confirmed the reciprocal differences in the interspecific hybridization between C. hystrix and C. sativus.

【关键词】 同工酶黄瓜种间杂种
【Key words】 isozymecucumberinterspecific hybrid.
【基金】 国家自然科学基金(30470120);国家“863”计划项目(2004AA241120);PickleSeedResearchFundofPicklePackerInternational,Inc.~~
  • 【文献出处】 植物分类学报 ,Acta Phytotaxonomica Sinica , 编辑部邮箱 ,2006年05期
  • 【分类号】Q943
  • 【被引频次】25
  • 【下载频次】206
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