【摘要】 隐地疫霉引起的根腐病是非洲菊生产上的主要病害,为发展该病的快速诊断技术,本文比较了卵菌核糖体基因ITS的序列,在此基础上设计了2条针对隐地疫霉的特异性PCR引物PC1和PC2。供试的23种不同真菌和疫霉菌的46个菌株中,利用这对引物能从隐地疫霉基因组DNA中扩增出一条分子量为620 bp的特异性条带,该引物的检测灵敏度可达10pg。采用快速组织碱裂解法提取发病植物组织的DNA,结合PCR检测技术,4 h内可从发病的非洲菊根部组织中特异性地检测到隐地疫霉菌。结果表明,建立的非洲菊疫霉根腐病菌分子检测方法可用于该病害的快速分子诊断。更多还原

【Abstract】 Root rot of Gerbera jamesonii caused by Phytophthora cryptogea is a serious disease in China, especially in wet condition.Since many pathogens were reported could infect the root of G.jamesonii and the isolation and identification of Phytophthora is really difficult,a rapid and accurate method for specific detection of P.cryptogea is necessary for determination of root rot in diseased G.jamesonii tissue.Based on the alignment of ITS sequences from Oomycetes,two primers were synthesized and a PCR based protocol was designed to detect and diagnostic of the G.jamesonii root rot caused by P.cryptogea specifically. Among 46 isolates representing 23 species of Oomycetes and fungi,a PCR product about 620 bp could only be amplified from P.cryptogea.The detection sensitivity is about 10 pg.The PC primer based PCR assay will provide a accurate and sensitivity tool for detection of P.cryptogea in G.jamesonii root rot.更多还原