【摘要】 目的建立微孔板杂交技术检测乙肝病毒X基因的方法,研究其在肝细胞癌诊断中的应用价值。方法在微孔板上包被HBV X基因片段的捕获探针;应用Bio-11-dUTP修饰的HBV X基因扩增引物,对待测标本进行PCR扩增,在微孔板上杂交后用链霉亲和素碱性磷酸酶系统显色。结果成功建立了微孔板杂交技术检测HBV X基因的方法;乙肝病毒感染后肝细胞癌患者X基因阳性检出率明显高于慢性乙肝患者和肝纤维化患者(P<0.01)。结论检测HBV X基因对肝细胞癌具有辅助诊断价值。更多还原

【Abstract】 Objective To set up hybridization technique on microwell plate to detect HBV X gene,invest to clinical application value on HCC.Methods On microwell plate capture probe was coated HBV X gene frangment;Gene which was modified by Bio-11-dUTP amplified primer.PCR amplified objective fragment of examined sample.After hybridization on microwell plate,using streptavidin ALP system colorated.Results It is successful to set up hybridization technique on microwell plate to detect HBV X.The method detected HBV X sensitiveness and idio;X gene of positive rate of HCC patients is obviously more than that of chronic serm hepatitis and hepatic fibrosis(P<0.01).Conclusion Detecting HBV X gene possess auxiliary diagnosis value for HCC.更多还原